Episode 4: I'm Ashwath, and this is my research blog

The meme this week is pretty fresh, only 20 days old. It's the I'm Rick Harrison and this is my pawn shop. I work here with my old man and my son, Big Hoss. Everything in here has a story and a price. One thing I’ve learned after 21 years – you never know WHAT is gonna come through that door. I don't understand myself why this is so funny, but the internet is going crazy replacing this classic intro with other people. I’m Harambe, and this is my zoo enclosure. I work here with my zoo keeper and my friend, Cecil the lion. Everything in here has a story and a price. One thing I’ve learned after 21 years – you never know WHO is gonna come over that fence. This cracks me up every time, but anyways back to AP Research. I've read more sources, so that's cool.

First, I'm Ashwath, and this is my research blog. I type here with my dax and my teacher, Mrs. Haag. Everything in here has a story and a meme. One thing I've learned after 4 weeks - you never know WHAT is gonna come through the works cited section. OK I'm done. But Literature reviews am I right? For my literature review, I need to set up the significance, any relevant information, and my gap in the research. So to build the significance of my protein research, I need to set up why finding a difference in conserved mutation function and non conserved mutation function matters. To show this I plan to talk about the history behind protein folding, and why it has always been a struggle to properly predict the folds a protein makes. Whether these mutations change the function (folds) can lead to further research techniques for large proteins like LAC1 in the future. There will probably have to be a large amount of defining or watering down in the literature review because a science background is practically required to decipher the research papers. But hey if I am able to understand it (which I am), I can translate into more layman terms. But as of now I don't know too many of the terms that need to be defined. Finding a gap in my research is relevant for A) moving towards a more efficient computer based prediction model over lab based procedure that are long and expensive and B) LAC1 which could help guide research in understanding the ways to fix the folding problems associated with its diseases. BUT the number 1 goal of my lit review is to have the reader understand what I am writing.

Second, I'm Ashwath, and this is ... OK I'm actually done. But an important source is probably the one from the Protein Data Base Databank describing the LAC1 protein function. This is probably the most important gap I have, which is the uniqueness of the LAC1 repressor. It is not only linked to specific diseases such and Lactose intolerance, it is also a large protein sequence, which computer simulations have struggle with. The source described the function of the genes that LAC1 protein represses. The genes code for proteins that break lactose into glucose, galactose, and other sugars. The LAC1 repressor prevents the creation of Beta-galactosidase, which is the enzyme that performs the first step in lactose metabolism, making glucose and galactose. It also doesn't produce Lactose permease transports lactose through membranes. These details describe its function. The crystal structure of the lac repressor is a bent structure with all 4 of the DNA binding portions pointing in one direction, showing the largeness of the structure, 333 amino acids long. So yeah this source is pretty useful. This source doesn't have a real academic conversation surrounding it, but there is more conversation with computer simulations vs. lab results, and conserved/non-conserved positions existing or not. That is where the other gaps will come, however I think this is the strongest gap I have and thus I talked about it. I have the sources describing my methods and the computer simulations on the way, so I'll get some more controversial foundational sources soon.

Third, I'm John Oliver and this is my comedy show. I work here with my writers and my inspiration, Harambe. Everything in here has a story and a price. One thing I’ve learned after 2 years – you never know WHAT is gonna be corrupted. Ok actually I'm done. I love John Oliver and I watch him every Monday morning, even though I don't agree with him every time. Discussing this baller in class is chill with me, although I hope we don't get too hung up on him to keep paper on time. I watched the charter school segment and I agree with Oliver on most points, but I am happy to argue from a BASIS point of view.

Fourth, IM BILLY MAYES AND THIS IS MY OXI-CLEAN STAND. I WORK HERE WITH MY OLD MAN, MR. CLEAN, AND MY SON, BARRY B. BENSON. EVERYTHING HERE HAS STAIN REMOVING POWERS.. AND A LARGE PRICE. ONE THING IVE LEARNED AFTER 21 YEARS- YOU NEVER KNOW WHAT LIQUID WILL RUIN YOUR CARPET/CLOTHES/ETC NEXT. Actually I ran this meme into the ground at this point. (871(theres defn 400 relevant words in here i promise))