So today on the meme enlightenment blog with some research on the side, we got a real classic here, Gnome Child, or Slayer. From the old Runescape days, the slayer arises into the Dank Meme mascot we all love and know today. Andy Salad has a terrible song with this Gnome Child. But on the topic of subtopics, dividing my area of inquiry is a difficult task.
This week I explored why LAC1 repressor, what does that even mean? Should I even care about it? That was basically this weekend. So LAC1 is just a protein that binds to DNA to prevent the coding of 3 proteins. Beta-galactosidase, which is the enzyme that performs the first step in lactose metabolism, making glucose and galactose. Galactoside acetyltransferase, which actually doesn't really do much in lactose metabolism (its unclear). And lactose permease transports lactose through membranes. LAC1 is called a repressor, because if it binds correctly lactose breakdown is stopped. But if the binding is off, the proteins are created and lactose is digested. The genes used for lactose utilization are negatively regulated. LAC1 binds and prevents the transcription of the genes that are used for lactose utilization. Also the protein is made of 4 subunits, and one side of the protein is a mirror of the other.
In terms of the subtopics, I don't really have a guided opinion of where to break the subtopics. In the papers, its written in english but you know its kinda not written in english with all the scientific terms so it takes forever to read through the literature. But I have vague idea. One subtopic would be the function of LAC1 and why its important. Another would be protein structure prediction models. Another would be the methods associated with testing. But I am not sure about the subtopics in my particular field. I need help here the most. For know I just looked into LAC1. I choose these topics because they are the most pertinent to the paper, why am I using this protein? what will this project be using? why look into protein structures?
I think my paper will be based around the history of folding proteins, and the significance of determining whether folding is altered by certain residue positions in LAC1. To end the blog I'll bring back the classic Gnome child in this beautiful track by Andy Salad.
https://www.youtube.com/watch?v=ldN9fNhZcsQ
Ashwath, as you move forward, I definitely want to see more clarity in terms of what your literature review needs. From my limited knowledge (as in, I may sound entirely like a n00b), you're right, you'll need to establish why this protein. I think, though, in order to figure out your methods, you'll need to talk about various mutations that may impact the protein folding (again, I may sound like an idiot, but I'm trying). Does that make sense?
ReplyDeleteWoah Ashwath the memes are too dank I'm impressed, but in all seriousness, I think that you have a good grasp on what you're going to do, you just don't know why it's important that you're doing it. So I think you can kind of open with a subtopic about lactose intolerance and the health effects that has/how many people it affects/other possible health effects it could lead to and then transition into explaining the structure and function of the LAC operon (trying to make it as common-sensey as possible for us because let's be real no one wants to try and get back into AP Bio brain mode) and then explain the significance of what you're doing/how you're testing/what it can reveal. Then again, I'm still kind of confused about all that bio stuff so I might be spewing nonsense, but do you kind of get my vibe? Overall, I think though sifting through all that scientific stuff sounds pretty tough, you've got to do it. Good luck!! I'm sure it won't be as bad as it seems!
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Wow what an old school, classic meme, you truly are the meme master, the human embodiment of "Know Your Meme". Should you be proud or ashamed? Should I be disgusted or impressed? I am not really sure and that dank video really made me question if you are okay, but now, like your blogpost, I will make a "seamless" transition into my comment. I thought Daphne presented some great ideas in terms how to break it up! By first focusing on your significance and the real-life health applications of research, so who is affected by the irregular operation of LAC, the reader can understand its significance right away, which is important especially in science research papers. You can then go into the function of lac operon, and like you mentioned the history of its research/past research discoveries. And I have to agree with Mrs. Haag, Daphne, and even yourself that your topics will "unfold" (hahah like a protein get it) the more research you do and the better you master the jargon. I am sure there are more specific ways to break up your complex research just based on how complicated the LAC operon sounds, its genes/mutations, and its role in a negative feedback loop that is essential to life. Um, good luck! Hopefully your memes will give you comfort through the arduous months ahead.
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DeleteBreak it up by the mutation points maybe. Establish of significance and function. Lactose intolerance and this leads to more research. Break it down by structure. Maybe you should read the 4 god papers. Kinda cut off rip harambe
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