Welcome back and enjoy the memes boys. Let's start off with an in depth analysis of DJ Khaled and his glorious sayings. The famous producer has been called out for his hip memes and in depth culture. One of his famous sayings is Anotha One. He reminds people that he is here to stay and he will always drop fire tracks as long as he is alive. Lion is another gem. It represents his commitment to being number 1 and being ferocious. Of course his famous saying Major Key is his most prominent saying. He refers to his most important things with Major Key alert. And if you have heard any song with DJ Khaled, you better believe he's screaming DJ KHALED.
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| When you want more trials. |
Back to the research project. In continuation from last week, I have in fact created a few chimeras for the protein I plan to analyze in February. Basically I searched more databases in an attempt to fin proper alternative sequences to code like Galactose. have collected the Lac1 PDB files and trimmed them into usable sequences. I probably will still have to rely on Paul, buff dope grad student (did I mention he's cool?) to get me the other chimera sequences as I have only found the ones normally available online. Finding these sequences are a still difficult.
Also I have determined that there is a high possibility that I am able to run trials simultaneously. So my analysis should complete at the same time in the process. The only things holding me back are computer errors, and the trials should be completed relatively easily. The timeline is still on pace, and I will probably have the samples to fire off by February 6th. Since I won't really fall behind on the timeline, I need to hit the ground running so I don't get cocky.
In terms of the majority of my work this week, I have decided to reorganize the literature review and align it in a more understandable way. The reorganization effort was difficult, but necessary for the future success of the project. The conservation section was moved from the structure of proteins to the computer analysis section, allowing for a more fluid flow of information between topics.
The fun part of the analysis is learning how to perform DFI trials and extracting the data. So I can't wait to get cracking on that. The vision is clear and I am ready to wreck this project. As DJ Khaled said, We the Best. And I am in the best state for this project. I am truly a LIONNNNNNNNN.
Hey 'Shwath!
ReplyDeleteAs you probably know, I love the meme of the week... but, I think you could raise your blog game by drawing some comparison, conversation, or insight from the meme of the week for your research. I don't know... just an idea (E.g., major key alert -- some proteins fold in a way where only specific "keys" can work as enzymes to unlock them). I also don't know biology, so that could just be wrong.
Anyways... I like that you are assembling these sequences and willing to reach out to our boi (yeah, now he's all of our classes' boi, not just yours) Paul. One question I had is, if you do you encounter a computer error, what measures do you foresee yourself taking to circumvent major delays? What types of errors are possible?
Regarding the lit review and methods, I like that you are increasing the conversation! What measures are you taking to make concepts like transcription and translation (i.e., the Central Dogma) understandable to a lay audience?
Always love your blog posts ... can't wait for anoth-a one!
Always LIOOOONNN order,
Yash
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Hey Ash-DJ KHALED-wath!
ReplyDeleteI think you've assembled the major key of your research (see what I did there)by getting all of your sequences assembled. I'm guessing yo boi Paul is totally willing to teach you what you need or at least rely on your stuff. I'm not sure exactly what the Chimera sequences are, if you could please explain that a bit better (remember, not a bio guy here). Which connects me to the point of remembering to make all your terms easy to understand for someone like me with limited bio knowledge. Furthermore, you mentioned the DFI trials again and I'm still not sure exactly how long those will take/amount of time it will take to analyze them. Will you have enough time?
See ya Shwath
Ashwath -- I still think you assume a lot of knowledge on behalf of your readers. Especially now that the audience will be expanding for your blogs, it's imperative that you make it so that people unfamiliar with your project know what's going on.
ReplyDeleteMoreover, the writing is so sloppy it's borderline atrocious. I think you're great, but you have to be a little more careful about editing what you wrote before you hit "submit."
Overall, though, I'm glad to hear that you've maneuvered a way to compensate for any hiccups in your schedule.